Glucose is a sugar with the molecular formula C6H12O6. The name “glucose” (/ˈɡluːkoʊz/, /ˈɡluːkoʊs/) comes from the Greek word γλυκος, meaning “sweet wine, must”. The suffix “-ose” is a chemical classifier, denoting a carbohydrate. It is also known as grape sugar. With 6 carbon atoms, it is classed as a hexose, a sub-category of monosaccharides. α-D-glucose is one of the 16 aldose stereoisomers. The D-isomer (D-glucose), also known as dextrose, occurs widely in nature, but the L-isomer (L-glucose) does not. Glucose is made during photosynthesis from water and carbon dioxide, using energy from sunlight. The reverse of the photosynthesis reaction, which releases this energy, is a very important source of power for cellular respiration. Glucose is stored as a polymer, in plants as starch and in animals as glycogen, for times when the organism will need it. Glucose circulates in the blood of animals as blood sugar. Glucose can be obtained by hydrolysis of carbohydrates such as milk, cane sugar, maltose, cellulose, glycogen etc. It is however, manufactured by hydrolysis of cornstarch by steaming and diluting acid.
Source: Wikipedia
USP Monograph information : Dextrose
Glucose
USP Method for Dextrose using Benson Polymeric Column
Part No.:
Eluent:
Flow Rate:
Detection:
Temperature:
Sample Size:
Column Size:
Resin Type:
DI H2O
0.3 ml/min
RI @ 40 C
85 C
20 ul, 30 mg/ml
300 x 7.8 mm
8% Ca
2 – Maltose + Isomaltose
3 – Dextrose
4 – Fructose
The USP Monograph states in part:
The relative retention times for maltotriose, maltose, isomaltose, dextrose, and fructose are 0.7, 0.8, 0.8, 1.0, and 1.3, respectively. The retention time for dextrose is about 21 min.
Additional Chromatogram(s) using Benson Polymeric Columns
Part No.:
Eluent:
Flow Rate:
Detection:
Temperature:
Sample Size:
Column Size:
Resin Type:
DI H2O
0.4 ml/min
RI
80 C
20 ul, 30 mg/ml
300 x 7.8 mm
6% Ca
2 – Maltose
3 – Lactulose
4 – Glucose
5 – Xylose
6 – Arabinose
7 – Ribitol
8 – Arabitol
9 – Xylitol
Part No.:
Eluent:
Flow Rate:
Detection:
Temperature:
Sample Size:
Column Size:
Resin Type:
DI H2O
0.4 ml/min
RI
90 C
20 ul, 30 mg/ml
300 x 7.8 mm
6% Ca
2 – Maltose
3 – Lactulose
4 – Glucose
5 – Fructose
Part No.:
Eluent:
Flow Rate:
Detection:
Temperature:
Sample Size:
Column Size:
Resin Type:
DI H2O
0.5 ml/min
RI
80 C
20 ul, 30 mg/ml
300 x 7.8 mm
6% Ca
2 – DP4
3 – DP3
4 – Maltose
5 – Glucose
6 – Galactose
7 – Fructose
8 – Mannitol
9 – Sorbitol
Part No.:
Eluent:
Flow Rate:
Detection:
Temperature:
Sample Size:
Column Size:
Resin Type:
DI H2O
0.5 ml/min
RI
85 C
20 ul, 30 mg/ml
300 x 7.8 mm
6% Ca
2 – Maltose
3 – Glucose
4 – Mannose
5 – Fructose
6 – Ribitol
Part No.:
Eluent:
Flow Rate:
Detection:
Temperature:
Sample Size:
Column Size:
Resin Type:
DI H2O
0.4 ml/min
RI
Ambient
20 ul, 30 mg/ml
300 x 7.8 mm
6% H
2 – Maltose
3 – Glucose
4 – Fructose
5 – Glycerol
6 – Acetic Acid
7 – Methanol
8 – Ethanol
Part No.:
Eluent:
Flow Rate:
Detection:
Temperature:
Sample Size:
Column Size:
Resin Type:
15mM K2HPO4
0.5 ml/min
RI
85 C
20 ul, 30 mg/ml
300 x 7.8 mm
6% K
2 – Glucose
3 – Betaine
4 – Glucose
5 – Fructose
Part No.:
Eluent:
Flow Rate:
Detection:
Temperature:
Sample Size:
Column Size:
Resin Type:
DI H2O
0.4 ml/min
RI
80 C
20 ul, 30 mg/ml
300 x 7.8 mm
4% Ca
2 – DP8
3 – DP7
4 – DP6
5 – DP5
6 – DP4
7 – DP3
8 – Maltose
9 – Glucose
10 – Fructose
Part No.:
Eluent:
Flow Rate:
Detection:
Temperature:
Sample Size:
Column Size:
Resin Type:
DI H2O
0.5 ml/min
RI
80 C
20 ul, 30 mg/ml
300 x 7.8 mm
6% Na
2 – DP5
3 – DP4
4 – DP3
5 – Maltose
6 – Glucose
7 – Fructose
Part No.:
Eluent:
Flow Rate:
Detection:
Temperature:
Sample Size:
Column Size:
Resin Type:
0.5 mM H2SO4
0.6 ml/min
RI
50 C
20 ul, 30 mg/ml
250 x 7.8 mm
8% H
2 – Glucose
3 – Fructose
4 – Succinic Acid
5 – Lactic Acid
6 – Glycerol
7 – Acetic Acid
8 – Ethanol
Part No.:
Eluent:
Flow Rate:
Detection:
Temperature:
Sample Size:
Column Size:
Resin Type:
DI H2O
0.6 ml/min
RI
85 C
20 ul, 30 mg/ml
300 x 7.8 mm
8% Ca
2 – Maltose
3 – Glucose
4 – Mannose
5 – Fructose
6 – Ribitol
Part No.:
Eluent:
Flow Rate:
Detection:
Temperature:
Sample Size:
Column Size:
Resin Type:
DI H2O
0.4 ml/min
RI
85 C
20 ul, 30 mg/ml
100 x 7.8 mm
8% Ca
2 – Glucose
3 – Fructose
Part No.:
Eluent:
Flow Rate:
Detection:
Temperature:
Sample Size:
Column Size:
Resin Type:
DI H2O
0.5 ml/min
RI
35 C
20 ul, 30 mg/ml
300 x 7.8 mm
8% H
2 – Glucose
3 – Fructose
4 – Succinic Acid
5 – Glycerol
6 – Acetic Acid
7 – Methanol
8 – Ethanol
Part No.:
Eluent:
Flow Rate:
Detection:
Temperature:
Sample Size:
Column Size:
Resin Type:
DI H2O
0.6 ml/min
RI
80 C
20 ul, 30 mg/ml
300 x 7.8 mm
8% K
2 – Glucose
3 – Betaine
4 – Glucose
5 – Fructose
Part No.:
Eluent:
Flow Rate:
Detection:
Temperature:
Sample Size:
Column Size:
Resin Type:
DI H2O
0.4 ml/min
RI
80 C
20 ul, 30 mg/ml
300 x 7.8 mm
8% Na
2 – Glucose
3 – Fructose
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