Fermentation & Biomass Columns
Fermentation is a metabolic process that converts sugar to acids and gases, or alcohol. It occurs in yeast and bacteria, and also in oxygen-starved muscle cells, as in the case of lactic acid fermentation. Fermentation is also used more broadly to refer to the bulk growth of microorganisms on a growth medium, often with the goal of producing a specific chemical product. French microbiologist Louis Pasteur is often remembered for his insights into fermentation and its microbial causes. The science of fermentation is known as zymology.
Source: Wikipedia
Biomass is an industry term for getting energy by burning wood, and other organic matter. Burning biomass releases carbon emissions, around a quarter higher than burning coal, but has been classed as a “renewable” energy source in the EU and UN legal frameworks, because plants can be regrown. It has become popular among coal power stations, which switch from coal to biomass to comply with the law. Biomass most often refers to plants or plant-based materials that are not used for food or feed, and are specifically called lignocellulosic biomass. As an energy source, biomass can either be used directly via combustion to produce heat, or indirectly after converting it to various forms of biofuel. Conversion of biomass to biofuel can be achieved by different methods which are broadly classified into: thermal, chemical, and biochemical methods.
Source: Wikipedia
Determination of Sugars, Byproducts, and Degradation Products in Liquid Fraction Process Samples Carbohydrates make up a major portion of biomass samples. These carbohydrates are polysaccharides constructed primarily of glucose, xylose, arabinose, galactose, and mannose monomeric subunits. During certain pretreatments of biomass, a portion of these polysaccharides are hydrolyzed and soluble sugars are released into the liquid stream. This method is used to quantify the total amount of soluble carbohydrates released into solution as well as the amount of monomeric sugars released into solution. The soluble sugars in the liquid fraction of process samples can be quantified by HPLC with refractive index detection. If the sugars are present in oligomeric form further processing into their monomeric units is required prior to HPLC analysis. The liquid portion may also contain carbohydrate degradation products, such as HMF and furfural, as well as other components of interest, such as organic acids and sugar alcohols.
Chromatograms
BP-800 Analytical Column
Part No.:
Eluent:
Flow Rate:
Detection:
Temperature:
Sample Size:
Column Size:
8115-0
0.5 mM H2SO4
0.6 ml/min
RI
50 C
20 ul, 30 mg/ml
200 x 7.8 mm
1. Maltose
2. Glucose
3. Fructose
4. Succinic
5. Lactic
6. Glycerol
7. Acetic
8. Ethanol
BP-100 H Analytical Column
Part No.:
Eluent:
Flow Rate:
Detection:
Temperature:
Sample Size:
Column Size:
DI H2O
0.6 ml/min
RI
55 C
20 ul, 30 mg/ml
300 x 7.8 mm
2 – Succinic
3 – Lactic
4 – Glycerol
5 – Acetic
6 – Ethanol
7 – Furfural
8 – 5-(Hydroxymethyl)furfural
BP-700 H Analytical Column
Part No.:
Eluent:
Flow Rate:
Detection:
Temperature:
Sample Size:
Column Size:
DI H2O
0.6 ml/min
RI
55 C
20 ul, 30 mg/ml
300 x 7.8 mm
2 – Succinic
3 – Lactic
4 – Glycerol
5 – Acetic
6 – Ethanol
7 – Furfural
8 – 5-(Hydroxymethyl)furfural
BP-800 H Analytical Column
Part No.:
Eluent:
Flow Rate:
Detection:
Temperature:
Sample Size:
Column Size:
DI H2O
0.6 ml/min
RI
55 C
20 ul, 30 mg/ml
300 x 7.8 mm
2 – Succinic
3 – Lactic
4 – Glycerol
5 – Acetic
6 – Ethanol
7 – Furfural
8 – 5-(Hydroxymethyl)furfural
Part Numbers
Other column dimensions are available upon request. Contact customer service for pricing and availability.
Part # | Size | Description |
---|---|---|
8115-0 | 200 x 7.8 mm | BP-800 H Analytical Column |
1100-0 | 300 x 7.8 mm | BP-100 H Analytical Column |
7100-0 | 300 x 7.8 mm | BP-700 H Analytical Column |
7110-0 | 150 x 7.8 mm | BP-700 H Analytical Column |
8100-0 | 300 x 7.8 mm | BP-800 H Analytical Column |
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