Protect Your Expensive Analytical Column and Save Money!
Benson Polymeric recommends using a guard column installed between the injector and analytical column. The guard column will remove particles and impurities that would normally collect on the inlet frit or in the first portion of the analytical column.
Guard columns need to be replaced on a regular basis, but that time frame will be determined by the quality of your sample. You will notice an increase in back pressure and a decrease in efficiency as the guard loads up with contaminates.
Our guards are typically 50 x 4.6 mm and packed with the same resin as in the analytical column, but with a larger diameter bead size to reduce back pressure. Using the same resin actually gives a slightly better chromatographic result (see chromatograms below).
Comparison of sample on a BP-800 Ca Column, 300 x 7.8 mm, with and without a guard.
Flow Rate: 0.6ml/min
Temperature: 85C
Pressure: 180 psi
Retention | Total Plates | Asym 5% |
---|---|---|
9.14 | 9555 | 1.31 |
9.94 | 7514 | 1.24 |
11.52 | 5892 | 1.08 |
12.89 | 12951 | 1.00 |
13.87 | 10633 | 0.98 |
15.34 | 16232 | 0.94 |
Flow Rate: 0.6ml/min
Temperature: 85C
Pressure: 130 psi
Retention | Total Plates | Asym 5% |
---|---|---|
8.72 | 9560 | 1.17 |
9.48 | 7752 | 1.11 |
10.96 | 5041 | 1.08 |
12.28 | 12054 | 0.97 |
13.27 | 9527 | 0.96 |
14.68 | 15980 | 0.91 |
You must be logged in to post a comment.